ID   BFH12
AC   CVCL_JQ51
SY   Bovine Foetal Hepatocyte 12
DR   Wikidata; Q54796189
RX   PubMed=27071625;
RX   PubMed=30617848;
RX   PubMed=38528259;
RX   PubMed=38648904;
CC   Characteristics: Can be used for applications requiring a bovine hepatocyte-derived cell line with long cultivability and high reproducibility.
CC   Senescence: SV40-transformed but with a finite life span. Dies out during passages 40-45.
CC   Doubling time: 23.5 hours (Note=At 11th passage), 25.6 hours (Note=At 31th passage) (PubMed=27071625).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRetro-E2 SV40).
CC   Derived from site: In situ; Fetal liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
CC   Breed/subspecies: Holstein Friesian; VBO=VBO_0003171.
OX   NCBI_TaxID=9913; ! Bos taurus (Bovine)
SX   Sex unspecified
AG   Fetus
CA   Finite cell line
DT   Created: 22-08-17; Last updated: 10-04-25; Version: 11
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RX   PubMed=27071625; DOI=10.1007/s11626-016-0018-0;
RA   Gleich A., Kaiser B., Schumann J., Fuhrmann H.;
RT   "Establishment and characterisation of a novel bovine SV40 large
RT   T-antigen-transduced foetal hepatocyte-derived cell line.";
RL   In Vitro Cell. Dev. Biol. Anim. 52:662-672(2016).
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RX   PubMed=30617848; DOI=10.1007/s10616-018-0279-4; PMCID=PMC6368520;
RA   Gleich A., Kaiser B., Honscha W., Fuhrmann H., Schoeniger A.;
RT   "Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro
RT   model for bovine biotransformation.";
RL   Cytotechnology 71:231-244(2019).
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RX   PubMed=38528259; DOI=10.1007/s10565-024-09856-7; PMCID=PMC10963470;
RA   Iori S., D'Onofrio C., Laham-Karam N., Mushimiyimana I., Lucatello L.,
RA   Lopparelli R.M., Gelain M.E., Capolongo F., Pauletto M., Dacasto M.,
RA   Giantin M.;
RT   "Establishment and characterization of cytochrome P450 1A1 CRISPR/Cas9
RT   knockout bovine foetal hepatocyte cell line (BFH12).";
RL   Cell Biol. Toxicol. 40:18.1-18.15(2024).
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RX   PubMed=38648904; DOI=10.1016/j.bcp.2024.116231;
RA   Iori S., D'Onofrio C., Laham-Karam N., Mushimiyimana I., Lucatello L.,
RA   Montanucci L., Lopparelli R.M., Bonsembiante F., Capolongo F.,
RA   Pauletto M., Dacasto M., Giantin M.;
RT   "Generation and characterization of cytochrome P450 3A74 CRISPR/Cas9
RT   knockout bovine foetal hepatocyte cell line (BFH12).";
RL   Biochem. Pharmacol. 224:116231.1-116231.11(2024).
//