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Cellosaurus RT4-67 (CVCL_JK04)

[Text version]
Cell line name RT4-67
Synonyms RT4-D6P2T clone 67
Accession CVCL_JK04
Resource Identification Initiative To cite this cell line use: RT4-67 (RRID:CVCL_JK04)
Comments Characteristics: Expression of NF2 is doxycycline-inducible as the parent cell line was transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=11316791).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7773; NF2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: ChEBI; CHEBI_23995; N-ethyl-N-nitrosourea (ENU).
Derived from site: In situ; Peripheral nervous system, sciatic nerve; UBERON=UBERON_0001322.
Cell type: Schwann cell; CL=CL_0002573.
Disease Rat malignant schwannoma (NCIt: C60465)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: BDIX; RS=RS_0000091.
Hierarchy Parent: CVCL_4006 (RT4-D6P2T)
Category Cancer cell line
Publications

PubMed=11316791; DOI=10.1101/gad.189601; PMCID=PMC312675
Helen Morrison, Larry S. Sherman, James Legg, Fatima Banine, Clare Isacke, Carrie Ann Haipek, David Hillel Gutmann, Helmut Ponta, Peter Herrlich;
The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
Genes Dev. 15:968-980(2001)

PubMed=14580336; DOI=10.1016/S1097-2765(03)00382-4
Joseph L. Kissil, Erik W. Wilker, Kristen C. Johnson, Matthew S. Eckman, Michael Bruce Yaffe, Tyler Jacks;
Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1.
Mol. Cell 12:841-849(2003)

PubMed=21481793; DOI=10.1016/j.ccr.2011.02.017; PMCID=PMC3075552
Chun-Ling Yi, Scott Troutman, Daniela Fera, Anat O. Stemmer-Rachamimov, Jacqueline L. Avila, Neepa Christian, Nathalie Luna Persson, Akihiko Shimono, David W. Speicher, Ronen Marmorstein, Lars Holmgren, Joseph L. Kissil;
A tight junction-associated merlin-angiomotin complex mediates merlin's regulation of mitogenic signaling and tumor suppressive functions.
Cancer Cell 19:527-540(2011)

PubMed=28469964; PMCID=PMC5411799
Jian-Man Guo, Michael R. Grovola, Hong Xie, Grace E. Coggins, Patrick Duggan, Rukhsana Hasan, Jia-Le Huang, Danny W. Lin, Claire Song, Gabriela M. Witek, Simon Berritt ...Show all 13 authors... , David C. Schultz, Jeffrey Field; Show fewer authors
Comprehensive pharmacological profiling of neurofibromatosis cell lines.
Am. J. Cancer Res. 7:923-934(2017)

Cross-references
Encyclopedic resources Wikidata; Q54951361
Experimental variables resources EFO; EFO_0022785
Entry history
Entry creation15-May-2017
Last entry update14-Aug-2025
Version number12