ID   RT4-67
AC   CVCL_JK04
SY   RT4-D6P2T clone 67
DR   EFO; EFO_0022785
DR   Wikidata; Q54951361
RX   PubMed=11316791;
RX   PubMed=14580336;
RX   PubMed=21481793;
RX   PubMed=28469964;
CC   Characteristics: Expression of NF2 is doxycycline-inducible as the parent cell line was transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=11316791).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7773; NF2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: ChEBI; CHEBI_23995; N-ethyl-N-nitrosourea (ENU).
CC   Derived from site: In situ; Peripheral nervous system, sciatic nerve; UBERON=UBERON_0001322.
CC   Cell type: Schwann cell; CL=CL_0002573.
CC   Breed/subspecies: BDIX; RS=RS_0000091.
DI   NCIt; C60465; Rat malignant schwannoma
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_4006 ! RT4-D6P2T
CA   Cancer cell line
DT   Created: 15-05-17; Last updated: 10-04-25; Version: 11
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RX   PubMed=11316791; DOI=10.1101/gad.189601; PMCID=PMC312675;
RA   Morrison H., Sherman L.S., Legg J., Banine F., Isacke C., Haipek C.A.,
RA   Gutmann D.H., Ponta H., Herrlich P.;
RT   "The NF2 tumor suppressor gene product, merlin, mediates contact
RT   inhibition of growth through interactions with CD44.";
RL   Genes Dev. 15:968-980(2001).
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RX   PubMed=14580336; DOI=10.1016/S1097-2765(03)00382-4;
RA   Kissil J.L., Wilker E.W., Johnson K.C., Eckman M.S., Yaffe M.B.,
RA   Jacks T.;
RT   "Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor
RT   of the p21-activated kinase, Pak1.";
RL   Mol. Cell 12:841-849(2003).
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RX   PubMed=21481793; DOI=10.1016/j.ccr.2011.02.017; PMCID=PMC3075552;
RA   Yi C.-L., Troutman S., Fera D., Stemmer-Rachamimov A.O., Avila J.L.,
RA   Christian N., Persson N.L., Shimono A., Speicher D.W., Marmorstein R.,
RA   Holmgren L., Kissil J.L.;
RT   "A tight junction-associated merlin-angiomotin complex mediates
RT   merlin's regulation of mitogenic signaling and tumor suppressive
RT   functions.";
RL   Cancer Cell 19:527-540(2011).
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RX   PubMed=28469964; PMCID=PMC5411799;
RA   Guo J.-M., Grovola M.R., Xie H., Coggins G.E., Duggan P., Hasan R.,
RA   Huang J.-L., Lin D.W., Song C., Witek G.M., Berritt S., Schultz D.C.,
RA   Field J.;
RT   "Comprehensive pharmacological profiling of neurofibromatosis cell
RT   lines.";
RL   Am. J. Cancer Res. 7:923-934(2017).
//