Cellosaurus logo
expasy logo

Cellosaurus NtG20.i7 (CVCL_JA65)

[Text][XML][JSON]
Cell line name NtG20.i7
Accession CVCL_JA65
Resource Identification Initiative To cite this cell line use: NtG20.i7 (RRID:CVCL_JA65)
Comments Population: Caucasian.
Virology: Supports hepatitis B virus (HBV) replication for up to 35 days with production of infectious HBV in the culture supernatant.
Knockout cell: Method=shRNA knockdown; HGNC; HGNC:29233; MAVS.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:10905; SLC10A1.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=28338936; DOI=10.1093/intimm/dxx012
Wan-Ling Yao, Sotaro Ikeda, Yuta Tsukamoto, Keiko Shindo, Yukie Otakaki, Mian Qin, Yoshikazu Iwasawa, Fumihiko Takeuchi, Yuki Kaname, Yu-Chi Chou, Chung-Ming Chang ...Show all 16 authors... , Koichi Watashi, Takaji Wakita, Takeshi Noda, Hiroki Kato, Takashi Fujita; Show fewer authors
Establishment of a human hepatocellular cell line capable of maintaining long-term replication of hepatitis B virus.
Int. Immunol. 29:109-120(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_JA65
Encyclopedic resources Wikidata; Q54931114
Entry history
Entry creation15-May-2017
Last entry update10-Apr-2025
Version number18