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Cellosaurus MCF-10A PTEN(-/-) (CVCL_F6LY)

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Cell line name MCF-10A PTEN(-/-)
Synonyms PTEN-/−
Accession CVCL_F6LY
Resource Identification Initiative To cite this cell line use: MCF-10A PTEN(-/-) (RRID:CVCL_F6LY)
Comments Characteristics: Homozygous PTEN knockout generated by targeted deletion of exon 2 (PubMed=19843859).
Characteristics: Not tumorigenic in nude-Foxn1nu mice (PubMed=26497685).
Knockout cell: Method=Homologous recombination; HGNC; HGNC:9588; PTEN.
Derived from site: In situ; Breast, epithelium; UBERON=UBERON_0008367.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_F6LX (MCF-10A PTEN(+/-))
Children:
CVCL_F6KS (MCF10A PTEN-/- KRas(G12V) clone 1)CVCL_F6KU (MCF10A PTEN-/- KRas(G12V) clone 2)CVCL_F6KY (PTEN-/-Luc)
Sex of cell Female
Age at sampling 36Y
Category Spontaneously immortalized cell line
Publications

PubMed=19843859; DOI=10.1158/0008-5472.CAN-09-1067; PMCID=PMC2783190
Michele I. Vitolo, Michele B. Weiss, Marta Szmacinski, Khola Tahir, Todd Waldman, Ben Ho Park, Stuart S. Martin, David J. Weber, Kurtis E. Bachman;
Deletion of PTEN promotes tumorigenic signaling, resistance to anoikis, and altered response to chemotherapeutic agents in human mammary epithelial cells.
Cancer Res. 69:8275-8283(2009)

PubMed=26497685; DOI=10.18632/oncotarget.6159; PMCID=PMC4742101
Keyata N. Thompson, Rebecca A. Whipple, Jennifer R. Yoon, Michael Lipsky, Monica S. Charpentier, Amanda E. Boggs, Kristi R. Chakrabarti, Lekhana Bhandary, Lindsay K. Hessler, Stuart S. Martin, Michele I. Vitolo;
The combinatorial activation of the PI3K and Ras/MAPK pathways is sufficient for aggressive tumor formation, while individual pathway activation supports cell persistence.
Oncotarget 6:35231-35246(2015)

Entry history
Entry creation22-Oct-2026
Last entry update22-Oct-2026
Version number1