ID   MCF-10A PTEN(-/-)
AC   CVCL_F6LY
SY   PTEN-/−
RX   PubMed=19843859;
RX   PubMed=26497685;
CC   Characteristics: Homozygous PTEN knockout generated by targeted deletion of exon 2 (PubMed=19843859).
CC   Characteristics: Not tumorigenic in nude-Foxn1nu mice (PubMed=26497685).
CC   Knockout cell: Method=Homologous recombination; HGNC; HGNC:9588; PTEN.
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Breast, epithelium; UBERON=UBERON_0008367.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_F6LX ! MCF-10A PTEN(+/-)
SX   Female
AG   36Y
CA   Spontaneously immortalized cell line
DT   Created: 22-10-26; Last updated: 22-10-26; Version: 1
CH   CVCL_F6KS ! MCF10A PTEN-/- KRas(G12V) clone 1
CH   CVCL_F6KU ! MCF10A PTEN-/- KRas(G12V) clone 2
CH   CVCL_F6KY ! PTEN-/-Luc
//
RX   PubMed=19843859; DOI=10.1158/0008-5472.CAN-09-1067; PMCID=PMC2783190;
RA   Vitolo, Michele I.
RA   Weiss, Michele B.
RA   Szmacinski, Marta
RA   Tahir, Khola
RA   Waldman, Todd
RA   Park, Ben Ho
RA   Martin, Stuart S.
RA   Weber, David J.
RA   Bachman, Kurtis E.
RT   "Deletion of PTEN promotes tumorigenic signaling, resistance to
RT   anoikis, and altered response to chemotherapeutic agents in human
RT   mammary epithelial cells.";
RL   Cancer Res. 69:8275-8283(2009).
//
RX   PubMed=26497685; DOI=10.18632/oncotarget.6159; PMCID=PMC4742101;
RA   Thompson, Keyata N.
RA   Whipple, Rebecca A.
RA   Yoon, Jennifer R.
RA   Lipsky, Michael
RA   Charpentier, Monica S.
RA   Boggs, Amanda E.
RA   Chakrabarti, Kristi R.
RA   Bhandary, Lekhana
RA   Hessler, Lindsay K.
RA   Martin, Stuart S.
RA   Vitolo, Michele I.
RT   "The combinatorial activation of the PI3K and Ras/MAPK pathways is
RT   sufficient for aggressive tumor formation, while individual pathway
RT   activation supports cell persistence.";
RL   Oncotarget 6:35231-35246(2015).
//