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Cellosaurus HeLa TRIP13 KO: auxin-inducible degron (AID)-TRIP13-Tet-Off. (CVCL_F4BW)

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Cell line name HeLa TRIP13 KO: auxin-inducible degron (AID)-TRIP13-Tet-Off.
Synonyms TRIP13 KO:AID; Clone 9; TRIP13KO AID-TRIP13
Accession CVCL_F4BW
Resource Identification Initiative To cite this cell line use: HeLa TRIP13 KO: auxin-inducible degron (AID)-TRIP13-Tet-Off. (RRID:CVCL_F4BW)
Comments Population: African American.
Characteristics: Expresses AID-TRIP13 under the control of a Tet-Off promoter in TRIP13 knockout background. Expression of AID-TRIP13 is suppress by doxycycline and indole-3-acetic acid (IAA).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:12307; TRIP13.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:12307; TRIP13 (Note=With a N-ter AID tag).
Genetic integration: Method=Transduction; Gene=UniProtKB; P93830; Arabidopsis thaliana IAA17 (Note=acts as an auxin-inducible degron (AID)).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q570C0; Arabidopsis thaliana TIR1 (Note=With a Myc tag).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2523 (HtTA-1)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=29425500; DOI=10.1016/j.celrep.2018.01.027
Hoi Tang Ma, Randy Y.C. Poon;
TRIP13 functions in the establishment of the spindle assembly checkpoint by replenishing O-MAD2.
Cell Rep. 22:1439-1450(2018)

Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1