Cellosaurus MB135-iDUX4 (CVCL_F1JH)
| Cell line name | MB135-iDUX4 |
|---|---|
| Synonyms | MB135-iDUX4 clone #7 |
| Accession | CVCL_F1JH |
| Resource Identification Initiative | To cite this cell line use: MB135-iDUX4 (RRID:CVCL_F1JH) |
| Comments | Characteristics: Transduced with plasmid pCW57.1-DUX4-CA that contains a tetO driven codon altered DUX4 and a hPGK-puroR-T2A-rtTA, where reverse tetracycline-controlled transactivator (rtTA) is a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Will produces the codon-altered DUX4 in response to induction by doxycycline (PubMed=28171552). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:50800; DUX4 (Note=Codon-altered). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4. Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Thigh, quadriceps skeletal muscle; UBERON=UBERON_0004498. Cell type: Myoblast; CL=CL_0000056. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_F1IZ (MB135) |
| Sex of cell | Female |
| Age at sampling | Age unspecified |
| Category | Telomerase immortalized cell line |
| Publications | PubMed=28171552; DOI=10.1093/hmg/ddw271; PMCID=PMC6078597 |
| Entry history | |
| Entry creation | 27-Nov-2025 |
| Last entry update | 27-Nov-2025 |
| Version number | 1 |