ID   MB135-iDUX4
AC   CVCL_F1JH
SY   MB135-iDUX4 clone #7
RX   PubMed=28171552;
CC   Characteristics: Transduced with plasmid pCW57.1-DUX4-CA that contains a tetO driven codon altered DUX4 and a hPGK-puroR-T2A-rtTA, where reverse tetracycline-controlled transactivator (rtTA) is a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Will produces the codon-altered DUX4 in response to induction by doxycycline (PubMed=28171552).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:50800; DUX4 (Note=Codon-altered).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Thigh, quadriceps skeletal muscle; UBERON=UBERON_0004498.
CC   Cell type: Myoblast; CL=CL_0000056.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_F1IZ ! MB135
SX   Female
AG   Age unspecified
CA   Telomerase immortalized cell line
DT   Created: 27-11-25; Last updated: 27-11-25; Version: 1
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RX   PubMed=28171552; DOI=10.1093/hmg/ddw271; PMCID=PMC6078597;
RA   Jagannathan, Sujatha
RA   Shadle, Sean C.
RA   Resnick, Rebecca
RA   Snider, Lauren D.
RA   Tawil, Rabi Nabil
RA   van der Maarel, Silvere Maria
RA   Bradley, Robert K.
RA   Tapscott, Stephen Justice
RT   "Model systems of DUX4 expression recapitulate the transcriptional
RT   profile of FSHD cells.";
RL   Hum. Mol. Genet. 25:4419-4431(2016).
//