Cellosaurus cell line HepG2/STAT3 (CVCL

Cellosaurus HepG2/STAT3 (CVCL_F1AI)

Entry history
Cell line name	HepG2/STAT3
Accession	CVCL_F1AI
Resource Identification Initiative	To cite this cell line use: HepG2/STAT3 (RRID:CVCL_F1AI)
Comments	Population: Caucasian. Characteristics: Used to monitor the activation of the STAT3 signaling pathway. Sensitive to interleukin-6 (IL6) (PubMed=37315652). Characteristics: Transfected with the pGL4.47[luc2P/SIE/Hygro] plasmid that contains a synthetic firefly luciferase (luc2P) gene under the control of five copies of the SIS-inducible element (SIE) (PubMed=37315652). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase (Note=With optimized codon usage for mammalian expression and a PEST sequence = Luc2P). Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease	Hepatoblastoma (NCIt: C3728) Hepatoblastoma (ORDO: Orphanet_449)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0027 (Hep-G2)
Sex of cell	Male
Age at sampling	15Y
Category	Cancer cell line
Publications	PubMed=37315652; DOI=10.1016/j.jep.2023.116700 Lian-Li Ni, Yi-Hao Che, Hong-Mei Sun, Bo Wang, Mei-Yu Wang, Zi-Zhong Yang, Heng Liu, Huai Xiao, Da-Song Yang, Hui-Lin Zhu, Zhi-Bin Yang; The therapeutic effect of wasp venom (Vespa magnifica, Smith) and its effective part on rheumatoid arthritis fibroblast-like synoviocytes through modulating inflammation, redox homeostasis and ferroptosis. J. Ethnopharmacol. 317:116700.1-116700.15(2023)
Entry creation	27-Nov-2025
Last entry update	27-Nov-2025
Version number	1