| Cell line name |
HT29-MTX resistant (10 microMolar) |
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| Synonyms |
HT29-MTX resistant (10-5M) |
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| Accession |
CVCL_F0KN |
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| Resource Identification Initiative |
To cite this cell line use: HT29-MTX resistant (10 microMolar) (RRID:CVCL_F0KN) |
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| Comments |
Population: Caucasian.
Characteristics: Resistant to 10 muM of methotrexate (PubMed=2205381).
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
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| Sequence variations |
- Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
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| Disease |
Colon adenocarcinoma (NCIt: C4349) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_0320 (HT-29) |
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| Sex of cell |
Female |
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| Age at sampling |
44Y |
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| Category |
Cancer cell line |
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| STR profile |
Source(s): ECACC=15121711
Markers:
Run an STR similarity search on this cell line |
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| Publications | PubMed=2205381
Thecla Lesuffleur, Alain Barbat, Elisabeth Dussaulx, Alain Zweibaum;
Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells.
Cancer Res. 50:6334-6343(1990) |
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| Cross-references |
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| Cell line collections (Providers) |
ECACC; 15121711
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| Entry history |
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| Entry creation | 14-Aug-2025 |
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| Last entry update | 14-Aug-2025 |
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| Version number | 1 |
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