Cellosaurus Gamma-ENaC-TetOn-mCCD (CVCL_E9V3)
| Cell line name | Gamma-ENaC-TetOn-mCCD |
|---|---|
| Accession | CVCL_E9V3 |
| Resource Identification Initiative | To cite this cell line use: Gamma-ENaC-TetOn-mCCD (RRID:CVCL_E9V3) |
| Comments | Characteristics: Conditionally overexpresses Scnn1g in response to doxycycline (PubMed=24179170). Characteristics: Transfected with two constructs, one is the reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and the other a pTet(tight)-Snn1g-IRES-HygR construct (PubMed=24179170). Genetic integration: Method=Transfection; Gene=MGI; MGI:104695; Scnn1g. Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Derived from site: In situ; Kidney, cortical collecting duct; UBERON=UBERON_0004203. Cell type: Kidney collecting duct principal cell; CL=CL_1001431. |
| Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) |
| Hierarchy | Parent: CVCL_B7HR (mCCDcl1) |
| Sex of cell | Male |
| Age at sampling | Age unspecified |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=24179170; DOI=10.1681/ASN.2013040429 |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |