ID   Gamma-ENaC-TetOn-mCCD
AC   CVCL_E9V3
RX   PubMed=24179170;
CC   Characteristics: Conditionally overexpresses Scnn1g in response to doxycycline (PubMed=24179170).
CC   Characteristics: Transfected with two constructs, one is the reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and the other a pTet(tight)-Snn1g-IRES-HygR construct (PubMed=24179170).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:104695; Scnn1g.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Derived from site: In situ; Kidney, cortical collecting duct; UBERON=UBERON_0004203.
CC   Cell type: Kidney collecting duct principal cell; CL=CL_1001431.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_B7HR ! mCCDcl1
SX   Male
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
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RX   PubMed=24179170; DOI=10.1681/ASN.2013040429;
RA   Wang, Yu-Bao
RA   Leroy, Valerie
RA   Maunsbach, Arvid Bernhard
RA   Doucet, Alain
RA   Hasler, Udo
RA   Bernabeu-Dizin, Eva
RA   Ernandez, Thomas
RA   de Seigneux, Sophie
RA   Martin, Pierre-Yves
RA   Feraille, Eric
RT   "Sodium transport is modulated by p38 kinase-dependent cross-talk
RT   between ENaC and Na,K-ATPase in collecting duct principal cells.";
RL   J. Am. Soc. Nephrol. 25:250-259(2014).
//