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Cellosaurus mCCDcl1 (CVCL_B7HR)

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Cell line name mCCDcl1
Synonyms mCCD(cl1); mCCD-CL1; mCCD cl1; mouse Cortical Collecting Duct clone 1
Accession CVCL_B7HR
Resource Identification Initiative To cite this cell line use: mCCDcl1 (RRID:CVCL_B7HR)
Comments Characteristics: Expresses epithelial sodium channels (ENaC) as well as the necessary cellular machinery to enable their stimulation by physiological concentrations of aldosterone, including 11-betahydroxysteroid dehydrogenase (HSD11b2), mineralocorticoid and glucocorticoid receptors and have therefore been used as a model for the study of principal cells physiology (PubMed=15743993; PubMed=29357433).
Doubling time: ~24 hours (Direct_author_submission).
Omics: Transcriptomics; RNAseq.
Miscellaneous: Doubling time from personal communication of Rossier, Bernard Claude.
Miscellaneous: Sex of mouse and STR profile from personal communication of Hummler, Edith.
Derived from site: In situ; Kidney, cortical collecting duct; UBERON=UBERON_0004203.
Cell type: Kidney collecting duct principal cell; CL=CL_1001431.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Children:
CVCL_E9V3 (Gamma-ENaC-TetOn-mCCD)CVCL_B7HS (mCCDcl1 Ed1)CVCL_B7HT (mCCDcl1 Ed2)
CVCL_B7HU (mCCDcl1 Ed3)
Sex of cell Male
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
STR profile Source(s): ABM=T0620; Direct_author_submission

Markers:
Mouse STR 1-116
Mouse STR 1-219,20
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-220.3
Mouse STR 5-513,14
Mouse STR 6-417.3 (Direct_author_submission)
18 (ABM=T0620)
Mouse STR 6-717
Mouse STR 7-126.2,27.2,31
Mouse STR 8-116
Mouse STR 11-217
Mouse STR 12-117
Mouse STR 13-117
Mouse STR 15-322.3
Mouse STR 17-215,17
Mouse STR 18-316
Mouse STR 19-212,13
Mouse STR X-128

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Publications

PubMed=15743993; DOI=10.1681/ASN.2004121110
Hans-Peter Gaeggeler, Elena Gonzalez-Rodriguez, Nicole Fowler-Jaeger, Dominique Loffing-Cueni, Rikke Norregaard, Johannes Loffing, Jean-Daniel Horisberger, Bernard Claude Rossier;
Mineralocorticoid versus glucocorticoid receptor occupancy mediating aldosterone-stimulated sodium transport in a novel renal cell line.
J. Am. Soc. Nephrol. 16:878-891(2005)

PubMed=19297448; DOI=10.1152/ajprenal.90475.2008
Heidi Fodstad, Elena Gonzalez-Rodriguez, Sylvian Bron, Hans-Peter Gaeggeler, Barbara Guisan, Bernard Claude Rossier, Jean-Daniel Horisberger;
Effects of mineralocorticoid and K+ concentration on K+ secretion and ROMK channel expression in a mouse cortical collecting duct cell line.
Am. J. Physiol. Renal Physiol. 296:F966-F975(2009)

PubMed=21178974; DOI=10.1038/ki.2010.486
Hans-Peter Gaeggeler, Yann Guillod, Dominique Loffing-Cueni, Johannes Loffing, Bernard Claude Rossier;
Vasopressin-dependent coupling between sodium transport and water flow in a mouse cortical collecting duct cell line.
Kidney Int. 79:843-852(2011)

PubMed=29357433; DOI=10.1152/ajprenal.00354.2017
Adrienne M. Assmus, Morag K. Mansley, Linda J. Mullins, Audrey Peter, John J. Mullins;
mCCDcl1 cells show plasticity consistent with the ability to transition between principal and intercalated cells.
Am. J. Physiol. Renal Physiol. 314:F820-F831(2018)

Cross-references
Cell line collections (Providers) ABM; T0620
Encyclopedic resources Wikidata; Q112930084
Entry history
Entry creation23-Jun-2022
Last entry update10-Apr-2025
Version number6