Cellosaurus RUESe002-A-6 (CVCL_E9CV)
| Cell line name | RUESe002-A-6 |
|---|---|
| Synonyms | RUES2-GLR |
| Accession | CVCL_E9CV |
| Resource Identification Initiative | To cite this cell line use: RUESe002-A-6 (RRID:CVCL_E9CV) |
| Comments | Registration: Swiss research registry; BAG-hES-IMP-0058. Characteristics: Using CRISPR/Cas9 three independent fate reporters SOX2-mCitrine, SOX17-tdTomato and TBXT-mCerulean were produced. First a P2A-H2B-tdTomato construct was inserted at the C-terminus of SOX17, then a PA2-mCerulean was inserted at the C-terminus of TBXT (called BRA) and finally a mCitrine was fused to the C-terminus of SOX2 (PubMed=29795348). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; J2JWA; mCerulean (Note=Bright cyan fluorescent protein derived from GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 3Q37R; mCitrine (Note=Yellow fluorescent protein derived from GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P33967; Bacillus cereus Bsr. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_B810 (RUES2) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=29795348; DOI=10.1038/s41586-018-0150-y; PMCID=PMC6077985 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; RUESe002-A-6 |
| Biological sample resources | BioSamples; SAMEA112765832 |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |