ID   RUESe002-A-6
AC   CVCL_E9CV
SY   RUES2-GLR
DR   BioSamples; SAMEA112765832
DR   hPSCreg; RUESe002-A-6
RX   PubMed=29795348;
CC   Registration: Swiss research registry; BAG-hES-IMP-0058.
CC   Characteristics: Using CRISPR/Cas9 three independent fate reporters SOX2-mCitrine, SOX17-tdTomato and TBXT-mCerulean were produced. First a P2A-H2B-tdTomato construct was inserted at the C-terminus of SOX17, then a PA2-mCerulean was inserted at the C-terminus of TBXT (called BRA) and finally a mCitrine was fused to the C-terminus of SOX2 (PubMed=29795348).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; J2JWA; mCerulean (Note=Bright cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 3Q37R; mCitrine (Note=Yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B810 ! RUES2
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
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RX   PubMed=29795348; DOI=10.1038/s41586-018-0150-y; PMCID=PMC6077985;
RA   Martyn, Iain
RA   Nakamura Kanno, Tatiane Yumi
RA   Ruzo, Albert
RA   Siggia, Eric Dean
RA   Brivanlou, Ali H.
RT   "Self-organization of a human organizer by combined Wnt and Nodal
RT   signalling.";
RL   Nature 558:132-135(2018).
//