Cellosaurus cell line MT1 (CVCL

Cellosaurus MT1 (CVCL_E8BN)

Cross-references
Cell line name	MT1
Accession	CVCL_E8BN
Resource Identification Initiative	To cite this cell line use: MT1 (RRID:CVCL_E8BN)
Comments	Population: Caucasian. Characteristics: Able to survive the cytotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Sensitive to mutagenesis by MNNG and display a mutator phenotype (PubMed=3745200). Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). Transformant: ChEBI; CHEBI_132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191). Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Omics: Transcriptomics; Microarray. Derived from site: In situ; Spleen; UBERON=UBERON_0002106. Cell type: B-cell; CL=CL_0000236.
Sequence variations	Mutation; HGNC; HGNC:11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (PubMed=33198927). Mutation; HGNC; HGNC:11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=33198927).
Disease	Hereditary spherocytosis (NCIt: C97074) Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0561 (TK6)
Sex of cell	Male
Age at sampling	5Y
Category	Transformed cell line
Publications	PubMed=3745200; DOI=10.1016/S0021-9258(18)67110-8 Victor S. Goldmacher, Robert A. Cuzick Jr., William G. Thilly; Isolation and partial characterization of human cell mutants differing in sensitivity to killing and mutation by methylnitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. J. Biol. Chem. 261:12462-12471(1986) PubMed=8341649; DOI=10.1073/pnas.90.14.6424; PMCID=PMC46944 Alexandra G. Kat, William G. Thilly, Woei-Horng Fang, Matthew J. Longley, Guo-Min Li, Paul Lawrence Modrich; An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair. Proc. Natl. Acad. Sci. U.S.A. 90:6424-6428(1993) PubMed=18805990; DOI=10.1101/gad.1688508; PMCID=PMC2559901 Rebecca C. Fry, J. Peter Svensson, Chandni Valiathan, Emma Wang, Brad J. Hogan, Sanchita Bhattacharya, James M. Bugni, Charles A. Whittaker, Leona D. Samson; Genomic predictors of interindividual differences in response to DNA damaging agents. Genes Dev. 22:2621-2626(2008)
Gene expression databases	GEO; GSM260649 GEO; GSM260650 GEO; GSM260651 GEO; GSM260652
Entry history
Entry creation	10-Apr-2025
Last entry update	10-Apr-2025
Version number	1