Cellosaurus cell line MT1 (CVCL

Cellosaurus MT1 (CVCL_E8BN)

Cross-references
Cell line name	MT1
Accession	CVCL_E8BN
Resource Identification Initiative	To cite this cell line use: MT1 (RRID:CVCL_E8BN)
Comments	Population: Caucasian. Characteristics: Able to survive the cytotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Sensitive to mutagenesis by MNNG and display a mutator phenotype (PubMed=3745200). Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). Transformant: ChEBI; CHEBI_132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191). Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Omics: Transcriptomics; Microarray. Derived from site: In situ; Spleen; UBERON=UBERON_0002106. Cell type: B-cell; CL=CL_0000236.
Sequence variations	Mutation; HGNC; HGNC:11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (PubMed=33198927). Mutation; HGNC; HGNC:11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=33198927).
Disease	Hereditary spherocytosis (NCIt: C97074) Hereditary spherocytosis (ORDO: Orphanet_822)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0561 (TK6)
Sex of cell	Male
Age at sampling	5Y
Category	Transformed cell line
Publications	PubMed=3745200; DOI=10.1016/S0021-9258(18)67110-8 Goldmacher V.S., Cuzick R.A. Jr., Thilly W.G. Isolation and partial characterization of human cell mutants differing in sensitivity to killing and mutation by methylnitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. J. Biol. Chem. 261:12462-12471(1986) PubMed=8341649; DOI=10.1073/pnas.90.14.6424; PMCID=PMC46944 Kat A.G., Thilly W.G., Fang W.-H., Longley M.J., Li G.-M., Modrich P.L. An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair. Proc. Natl. Acad. Sci. U.S.A. 90:6424-6428(1993) PubMed=18805990; DOI=10.1101/gad.1688508; PMCID=PMC2559901 Fry R.C., Svensson J.P., Valiathan C., Wang E., Hogan B.J., Bhattacharya S., Bugni J.M., Whittaker C.A., Samson L.D. Genomic predictors of interindividual differences in response to DNA damaging agents. Genes Dev. 22:2621-2626(2008)
Gene expression databases	GEO; GSM260649 GEO; GSM260650 GEO; GSM260651 GEO; GSM260652
Entry history
Entry creation	10-Apr-2025
Last entry update	10-Apr-2025
Version number	1