Cellosaurus cell line X4-MaRBLE (CVCL

Cellosaurus X4-MaRBLE (CVCL_E8AV)

[Text version]

Cell line name

X4-MaRBLE

Synonyms

HPB-Ma/LTR-FL/CMV-RL

Accession

CVCL_E8AV

Resource Identification Initiative

To cite this cell line use: X4-MaRBLE (RRID:CVCL_E8AV)

Comments

Population: Japanese.
Characteristics: Reporter cell line to measure HIV-1 infectivity and thus evaluate susceptibility to anti-HIV-1 drugs (PubMed=17182760).
Characteristics: Transfected with two constructs. 1) 53LTRN-lucneoR#1 which contains the HIV-1 strain HXB2 LTR driving the expression of a Luc-DsRed fusion protein. This allows HIV-1 replication to be quantitatively evaluated by using luciferase activity when the LTR is activated by Tat, and the DsRed gene allows HIV-1 infected cells to be identified by red fluorescence. 2) pRenillaPac where Renilla luciferase is under the control of a CMV promoter and is used to monitor cell numbers and viability (PubMed=17182760).
Virology: Infected with Moloney murine leukemia virus (MoMuLV) from MiCl1 (S+L-) (Cellosaurus=CVCL_3405) cells (from parent cell line).
Virology: Susceptible to infection by CXCR4-tropic HIV-1 strains (example HXB2) (PubMed=17182760).
Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.

Sequence variations

Gene fusion; HGNC; HGNC:1539; CBFB + HGNC; HGNC:29824; MYL11; Name(s)=CBFB-MYL11, CBFB-MYLPF (from parent cell line).
Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1574Pro (c.4721T>C); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Asp2442fs*39 (c.7326_7326insGGGCCGTGGACG); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys124Ter (c.372C>A); ClinVar=VCV000458537; Zygosity=Homozygous (from parent cell line).

Disease

Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_E8AT (HPB-Ma)
Children:

CVCL_E8AW (R5-MaRBLE)

Sex of cell

Male

Age at sampling

14Y