ID   X4-MaRBLE
AC   CVCL_E8AV
SY   HPB-Ma/LTR-FL/CMV-RL
RX   PubMed=17182760;
CC   Population: Japanese.
CC   Characteristics: Reporter cell line to measure HIV-1 infectivity and thus evaluate susceptibility to anti-HIV-1 drugs (PubMed=17182760).
CC   Characteristics: Transfected with two constructs. 1) 53LTRN-lucneoR#1 which contains the HIV-1 strain HXB2 LTR driving the expression of a Luc-DsRed fusion protein. This allows HIV-1 replication to be quantitatively evaluated by using luciferase activity when the LTR is activated by Tat, and the DsRed gene allows HIV-1 infected cells to be identified by red fluorescence. 2) pRenillaPac where Renilla luciferase is under the control of a CMV promoter and is used to monitor cell numbers and viability (PubMed=17182760).
CC   Virology: Infected with Moloney murine leukemia virus (MoMuLV) from MiCl1 (S+L-) (Cellosaurus=CVCL_3405) cells (from parent cell line).
CC   Virology: Susceptible to infection by CXCR4-tropic HIV-1 strains (example HXB2) (PubMed=17182760).
CC   Sequence variation: Gene fusion; HGNC; HGNC:1539; CBFB + HGNC; HGNC:29824; MYL11; Name(s)=CBFB-MYL11, CBFB-MYLPF (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1574Pro (c.4721T>C); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Asp2442fs*39 (c.7326_7326insGGGCCGTGGACG); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys124Ter (c.372C>A); ClinVar=VCV000458537; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E8AT ! HPB-Ma
SX   Male
AG   14Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=17182760; DOI=10.1128/JCM.01708-06; PMCID=PMC1829063;
RA   Chiba-Mizutani T., Miura H., Matsuda M., Matsuda Z., Yokomaku Y.,
RA   Miyauchi K., Nishizawa M., Yamamoto N., Sugiura W.;
RT   "Use of new T-cell-based cell lines expressing two luciferase
RT   reporters for accurately evaluating susceptibility to anti-human
RT   immunodeficiency virus type 1 drugs.";
RL   J. Clin. Microbiol. 45:477-487(2007).
//