Cellosaurus R5-MaRBLE (CVCL_E8AW)
Cell line name | R5-MaRBLE |
---|---|
Accession | CVCL_E8AW |
Resource Identification Initiative | To cite this cell line use: R5-MaRBLE (RRID:CVCL_E8AW) |
Comments | Population: Japanese. Characteristics: Reporter cell line to measure HIV-1 infectivity and thus evaluate susceptibility to anti-HIV-1 drugs (PubMed=17182760). Characteristics: Transfected with three constructs. 1) 53LTRN-lucneoR#1 which contains the HIV-1 strain HXB2 LTR driving the expression of a Luc-DsRed fusion protein. This allows HIV-1 replication to be quantitatively evaluated by using luciferase activity when the LTR is activated by Tat, and the DsRed gene allows HIV-1 infected cells to be identified by red fluorescence. 2) pRenillaPac where Renilla luciferase is under the control of a CMV promoter and is used to monitor cell numbers and viability, 3) pCCR5/CEP4 which contains CCR5 under the control of a CMV promoter (PubMed=17182760). Virology: Infected with Moloney murine leukemia virus (MoMuLV) from MiCl1 (S+L-) (Cellosaurus=CVCL_3405) cells (from parent cell line). Virology: Susceptible to infection by both CCR5-tropic (example JRCSF) and CXCR4-tropic HIV-1 strains (PubMed=17182760). Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1606; CCR5. Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. Cell type: T-cell; CL=CL_0000084. |
Sequence variations |
|
Disease | Childhood T acute lymphoblastic leukemia (NCIt: C7953) Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_E8AV (X4-MaRBLE) |
Sex of cell | Male |
Age at sampling | 14Y |
Category | Cancer cell line |
Publications | PubMed=17182760; DOI=10.1128/JCM.01708-06; PMCID=PMC1829063 |
Entry history | |
Entry creation | 10-Apr-2025 |
Last entry update | 10-Apr-2025 |
Version number | 1 |