Cellosaurus H9-iCas9.SAM (CVCL_E7G6)
| Cell line name | H9-iCas9.SAM |
|---|---|
| Accession | CVCL_E7G6 |
| Resource Identification Initiative | To cite this cell line use: H9-iCas9.SAM (RRID:CVCL_E7G6) |
| Comments | Characteristics: Cell line with Dox-inducible CRISPR-based Synergistic Activation Mediator (SAM) system (PubMed=39676795). Characteristics: Using CRISPR/Cas9 a TRE3G-dCas9-VP64-P2A-MS2-p65-HSF1-T2A-mCherry-Insulator-TETon3G-EF1a-Neo construct was introduced in the AAVS1 safe harbor locus (PubMed=39676795). Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1. Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del). Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys). Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_9773 (WA09) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=39676795; DOI=10.3389/fcell.2024.1484955; PMCID=PMC11638181 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |