ID   H9-iCas9.SAM
AC   CVCL_E7G6
RX   PubMed=39676795;
CC   Characteristics: Cell line with Dox-inducible CRISPR-based Synergistic Activation Mediator (SAM) system (PubMed=39676795).
CC   Characteristics: Using CRISPR/Cas9 a TRE3G-dCas9-VP64-P2A-MS2-p65-HSF1-T2A-mCherry-Insulator-TETon3G-EF1a-Neo construct was introduced in the AAVS1 safe harbor locus (PubMed=39676795).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=39676795; DOI=10.3389/fcell.2024.1484955; PMCID=PMC11638181;
RA   Petazzi P., Gutierrez-Aguera F., Roca-Ho H., Castano J., Bueno C.,
RA   Alvarez N.M., Forrester L.M., Sevilla A., Fidanza A., Menendez P.;
RT   "Generation of an inducible dCas9-SAM human PSC line for endogenous
RT   gene activation.";
RL   Front. Cell Dev. Biol. 12:1484955.1-1484955.10(2024).
//