Cellosaurus CHO-SIINFEKL/H-2Kb (CVCL_E7G0)
| Cell line name | CHO-SIINFEKL/H-2Kb |
|---|---|
| Synonyms | CHO.SIINFEKL/H-2Kb |
| Accession | CVCL_E7G0 |
| Resource Identification Initiative | To cite this cell line use: CHO-SIINFEKL/H-2Kb (RRID:CVCL_E7G0) |
| Comments | Characteristics: Enables the large-scale and continuous batch production of soluble, fully folded and epitope-defined MHC class I molecules. Engineered to secrete peptide/MHC complexes that can be selectively purified in bulk using affinity chromatography, biotinylated, multimerized (via streptavidin) and the resulting reagent incorporated into immunologically relevant assays for detection of antigen-specific T-cells (PubMed=30347189). Characteristics: Using a sleeping beauty transposon system a SIINFEKL-linker-B2m-linker-H2K(b)-AviTag construct was integrated in the parent cell line (PubMed=30347189). Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:88127; B2m. Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:95904; H2-K1. Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_7183 (CHO-S) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Web pages | Provider; TTU; -; https://ttu.flintbox.com/technologies/48eb7349-649b-475b-85b9-759856124d72 |
| Publications | PubMed=30347189; DOI=10.1016/j.jim.2018.10.006; PMCID=PMC6322931 Patent=US20210380661 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |