ID   CHO-SIINFEKL/H-2Kb
AC   CVCL_E7G0
SY   CHO.SIINFEKL/H-2Kb
RX   Patent=US20210380661;
RX   PubMed=30347189;
WW   Provider; TTU; -; https://ttu.flintbox.com/technologies/48eb7349-649b-475b-85b9-759856124d72
CC   Characteristics: Enables the large-scale and continuous batch production of soluble, fully folded and epitope-defined MHC class I molecules. Engineered to secrete peptide/MHC complexes that can be selectively purified in bulk using affinity chromatography, biotinylated, multimerized (via streptavidin) and the resulting reagent incorporated into immunologically relevant assays for detection of antigen-specific T-cells (PubMed=30347189).
CC   Characteristics: Using a sleeping beauty transposon system a SIINFEKL-linker-B2m-linker-H2K(b)-AviTag construct was integrated in the parent cell line (PubMed=30347189).
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:88127; B2m.
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:95904; H2-K1.
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7183 ! CHO-S
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   Patent=US20210380661;
RA   Lowe D.B., Wooster A.L.;
RT   "Method to express, purify, and biotinylate eukaryotic cell-derived
RT   major histocompatibility complexes.";
RL   Patent number US20210380661, 09-Dec-2021.
//
RX   PubMed=30347189; DOI=10.1016/j.jim.2018.10.006; PMCID=PMC6322931;
RA   Wooster A.L., Anderson T.S., Lowe D.B.;
RT   "Expression and characterization of soluble epitope-defined major
RT   histocompatibility complex (MHC) from stable eukaryotic cell lines.";
RL   J. Immunol. Methods 464:22-30(2019).
//