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Cellosaurus THP-1 IQAD biosensor (CVCL_E7CQ)

[Text version]
Cell line name THP-1 IQAD biosensor
Synonyms THP-1 caspase-1 biosensor
Accession CVCL_E7CQ
Resource Identification Initiative To cite this cell line use: THP-1 IQAD biosensor (RRID:CVCL_E7CQ)
Comments Population: Japanese.
Characteristics: Transduced with a firefly luciferase reporter circularly permuted in which the N- and C-terminal domains are physically separated by a flexible hinge region containing a caspase-1 cleaving site (IQADSGPIG) (PubMed=31562211).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=Circ. permuted with an IQAD caspase-1 site).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
  • Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
Disease Childhood acute monocytic leukemia (NCIt: C9163)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0006 (THP-1)
Sex of cell Male
Age at sampling 1Y
Category Cancer cell line
Publications

PubMed=31562211; DOI=10.4049/jimmunol.1900619
Talley S., Kalinina O., Winek M.A., Paik W., Cannon A.R., Alonzo F. 3rd, Choudhry M.A., Knight K.L., Campbell E.M.
A caspase-1 biosensor to monitor the progression of inflammation in vivo.
J. Immunol. 203:2497-2507(2019)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1