ID   THP-1 IQAD biosensor
AC   CVCL_E7CQ
SY   THP-1 caspase-1 biosensor
RX   PubMed=31562211;
CC   Population: Japanese.
CC   Characteristics: Transduced with a firefly luciferase reporter circularly permuted in which the N- and C-terminal domains are physically separated by a flexible hinge region containing a caspase-1 cleaving site (IQADSGPIG) (PubMed=31562211).
CC   Sequence variation: Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
CC   Sequence variation: Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=Circ. permuted with an IQAD caspase-1 site).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
DI   NCIt; C9163; Childhood acute monocytic leukemia
DI   ORDO; Orphanet_514; Acute monoblastic/monocytic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0006 ! THP-1
SX   Male
AG   1Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
//
RX   PubMed=31562211; DOI=10.4049/jimmunol.1900619;
RA   Talley S., Kalinina O., Winek M.A., Paik W., Cannon A.R., Alonzo F. 3rd,
RA   Choudhry M.A., Knight K.L., Campbell E.M.;
RT   "A caspase-1 biosensor to monitor the progression of inflammation in
RT   vivo.";
RL   J. Immunol. 203:2497-2507(2019).
//