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Cellosaurus Abcam SK-HEP-1 S1PR1 KO (CVCL_E7C3)

[Text version]
Cell line name Abcam SK-HEP-1 S1PR1 KO
Accession CVCL_E7C3
Resource Identification Initiative To cite this cell line use: Abcam SK-HEP-1 S1PR1 KO (RRID:CVCL_E7C3)
Comments Problematic cell line: Misclassified. Parent cell line (SK-HEP-1) was originally described as originating from an adenocarcinoma of liver and thus classified as hepatocellular carcinoma. Later studies show that it most probably has arisen from endothelial cells.
Population: Caucasian.
Karyotypic information: Has lost chromosome Y (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:3165; S1PR1.
Miscellaneous: Custom made by Abcam for the experiments described in DOI=10.12688/f1000research.153244.3.
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Cell type: Endothelial cell; CL=CL_0000115.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
Disease Liver and intrahepatic bile duct epithelial neoplasm (NCIt: C7106)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0525 (SK-HEP-1)
Sex of cell Male
Age at sampling 52Y
Category Cancer cell line
Publications

DOI=10.12688/f1000research.153244.3
Riham Ayoubi, Maryam Fotouhi, Charles Alende, Sara Gonzalez Bolivar, Kathleen Southern, Carl Laflamme;
ABIF Consortium
NeuroSGC/YCharOS/EDDU Collaborative Group
A guide to selecting high-performing antibodies for S1PR1 (UniProt ID: P21453) for use in western blot, immunoprecipitation, and immunofluorescence.
F1000Research 13:792.1-792.18(2024)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1