ID   Abcam SK-HEP-1 S1PR1 KO
AC   CVCL_E7C3
RX   DOI=10.12688/f1000research.153244.3;
CC   Problematic cell line: Misclassified. Parent cell line (SK-HEP-1) was originally described as originating from an adenocarcinoma of liver and thus classified as hepatocellular carcinoma. Later studies show that it most probably has arisen from endothelial cells.
CC   Population: Caucasian.
CC   Karyotypic information: Has lost chromosome Y (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:3165; S1PR1.
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Miscellaneous: Custom made by Abcam for the experiments described in DOI=10.12688/f1000research.153244.3.
CC   Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Endothelial cell; CL=CL_0000115.
DI   NCIt; C7106; Liver and intrahepatic bile duct epithelial neoplasm
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0525 ! SK-HEP-1
SX   Male
AG   52Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   DOI=10.12688/f1000research.153244.3;
RA   Ayoubi, Riham
RA   Fotouhi, Maryam
RA   Alende, Charles
RA   Gonzalez Bolivar, Sara
RA   Southern, Kathleen
RA   Laflamme, Carl
RG   ABIF Consortium;
RG   NeuroSGC/YCharOS/EDDU Collaborative Group;
RT   "A guide to selecting high-performing antibodies for S1PR1 (UniProt ID:
RT   P21453) for use in western blot, immunoprecipitation, and
RT   immunofluorescence.";
RL   F1000Research 13:792.1-792.18(2024).
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