Cellosaurus EndoC-betaH5 HLA-A2 (CVCL_E7A7)
| Cell line name | EndoC-betaH5 HLA-A2 |
|---|---|
| Synonyms | EndoC-betaH5-HLA-A2; HLA-A2 EndoC-betaH5 |
| Accession | CVCL_E7A7 |
| Resource Identification Initiative | To cite this cell line use: EndoC-betaH5 HLA-A2 (RRID:CVCL_E7A7) |
| Comments | Characteristics: Combines T-cell killing attack with measuring the indirect impact on insulin secretion and function loss (Human Cell Design=BH5-A2-350). Characteristics: First transduced with two vectors pTRIP DeltaU3loxP-pY-TK-RIP405-SV40LT and pTRIP DeltaU3loxP-pY-TK-RIP405-SV40-hTERT where RIP405 is a beta cell specific rat insulin promoter and pY, the polyoma virus enhancer. Was then transduced with a Lv-RIP-CreERT2, treated with tamoxifen and gancyclovir thus removing the SV40LT and hTERT immortalizing genes as well as HSV-1 TK (from parent cell line). Characteristics: Transduced with a HLA-A2-IRES-PuroR construct (PubMed=37442376). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:4931; HLA-A (Note=Allele A*2). Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006. Cell type: Pancreatic beta cell; CL=CL_0000169. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_C7TU (EndoC-betaH5) |
| Sex of cell | Female |
| Age at sampling | 8FW |
| Category | Transformed cell line |
| Web pages | Provider; Human Cell Design; BH5-A2-350; https://www.humancelldesign.com/hla-a2-endoc-bh5/ |
| Publications | PubMed=37442376; DOI=10.1016/j.molmet.2023.101772; PMCID=PMC10407753 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 2 |