ID   EndoC-betaH5 HLA-A2
AC   CVCL_E7A7
SY   EndoC-betaH5-HLA-A2; HLA-A2 EndoC-betaH5
RX   PubMed=37442376;
WW   Provider; Human Cell Design; BH5-A2-350; https://www.humancelldesign.com/hla-a2-endoc-bh5/
CC   Characteristics: Combines T-cell killing attack with measuring the indirect impact on insulin secretion and function loss (Human Cell Design=BH5-A2-350).
CC   Characteristics: First transduced with two vectors pTRIP DeltaU3loxP-pY-TK-RIP405-SV40LT and pTRIP DeltaU3loxP-pY-TK-RIP405-SV40-hTERT where RIP405 is a beta cell specific rat insulin promoter and pY, the polyoma virus enhancer. Was then transduced with a Lv-RIP-CreERT2, treated with tamoxifen and gancyclovir thus removing the SV40LT and hTERT immortalizing genes as well as HSV-1 TK (from parent cell line).
CC   Characteristics: Transduced with a HLA-A2-IRES-PuroR construct (PubMed=37442376).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:4931; HLA-A (Note=Allele A*2).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006.
CC   Cell type: Pancreatic beta cell; CL=CL_0000169.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C7TU ! EndoC-betaH5
SX   Female
AG   8FW
CA   Transformed cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=37442376; DOI=10.1016/j.molmet.2023.101772; PMCID=PMC10407753;
RA   Blanchi B., Taurand M., Colace-Sauty C., Thomaidou S., Audeoud C., Fantuzzi F.,
RA   Sawatani T., Gheibi S., Sabadell-Basallote J., Boot F.W.J., Chantier T.,
RA   Piet A., Cavanihac C., Pilette M., Balguerie A., Olleik H., Carlotti F.,
RA   Ejarque M., Fex M., Mulder H., Cnop M., Eizirik D.L., Jouannot O.,
RA   Gaffuri A.-L., Czernichow P., Zaldumbide A., Scharfmann R., Ravassard P.;
RT   "EndoC-betaH5 cells are storable and ready-to-use human pancreatic
RT   beta cells with physiological insulin secretion.";
RL   Mol. Metab. 76:101772.1-101772.13(2023).
//