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Cellosaurus EndoC-betaH5 (CVCL_C7TU)

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Cell line name EndoC-betaH5
Synonyms EndoC-BH5
Accession CVCL_C7TU
Resource Identification Initiative To cite this cell line use: EndoC-betaH5 (RRID:CVCL_C7TU)
Comments Characteristics: Displays robust GLP-1 and GIP receptor activity associated with insulin content and glucose stimulated insulin secretion levels, close to those of native human beta cells (Human Cell Design).
Characteristics: First transduced with two vectors pTRIP DeltaU3loxP-pY-TK-RIP405-SV40LT and pTRIP DeltaU3loxP-pY-TK-RIP405-SV40-hTERT where RIP405 is a beta cell specific rat insulin promoter and pY, the polyoma virus enhancer. Was then transduced with a Lv-RIP-CreERT2, treated with tamoxifen and gancyclovir thus removing the SV40LT and hTERT immortalizing genes as well as HSV-1 TK (PubMed=37442376).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006.
Cell type: Pancreatic beta cell; CL=CL_0000169.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E7A7 (EndoC-betaH5 HLA-A2)
Sex of cell Female
Age at sampling 8FW
Category Transformed cell line
Web pages Provider; Human Cell Design; -; https://www.humancelldesign.com/endoc-bh5/
Publications

PubMed=37442376; DOI=10.1016/j.molmet.2023.101772; PMCID=PMC10407753
Bruno Blanchi, Marion Taurand, Claire Colace-Sauty, Sofia Thomaidou, Charlotte Audeoud, Federica Fantuzzi, Toshiaki Sawatani, Sevda Gheibi, Joan Sabadell-Basallote, Fransje W.J. Boot, Thibault Chantier ...Show all 28 authors... , Aline Piet, Charlotte Cavanihac, Marion Pilette, Adelie Balguerie, Hamza Olleik, Francoise Carlotti, Miriam Ejarque, Malin Fex, Hindrik Mulder, Miriam Cnop, Decio Laks Eizirik, Ouardane Jouannot, Anne-Lise Gaffuri, Paul Czernichow, Arnaud Zaldumbide, Raphael Scharfmann, Philippe Ravassard; Show fewer authors
EndoC-betaH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.
Mol. Metab. 76:101772.1-101772.13(2023)

Cross-references
Encyclopedic resources Wikidata; Q117704314
Gene expression databases GEO; GSM7029173
GEO; GSM7029174
GEO; GSM7029175
GEO; GSM7029176
GEO; GSM7029177
Entry history
Entry creation21-Mar-2023
Last entry update10-Apr-2025
Version number4