ID   EndoC-betaH5
AC   CVCL_C7TU
SY   EndoC-BH5
DR   GEO; GSM7029173
DR   GEO; GSM7029174
DR   GEO; GSM7029175
DR   GEO; GSM7029176
DR   GEO; GSM7029177
DR   Wikidata; Q117704314
RX   PubMed=37442376;
WW   Provider; Human Cell Design; -; https://www.humancelldesign.com/endoc-bh5/
CC   Characteristics: Displays robust GLP-1 and GIP receptor activity associated with insulin content and glucose stimulated insulin secretion levels, close to those of native human beta cells (Human Cell Design).
CC   Characteristics: First transduced with two vectors pTRIP DeltaU3loxP-pY-TK-RIP405-SV40LT and pTRIP DeltaU3loxP-pY-TK-RIP405-SV40-hTERT where RIP405 is a beta cell specific rat insulin promoter and pY, the polyoma virus enhancer. Was then transduced with a Lv-RIP-CreERT2, treated with tamoxifen and gancyclovir thus removing the SV40LT and hTERT immortalizing genes as well as HSV-1 TK (PubMed=37442376).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Transcriptomics; RNAseq.
CC   Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006.
CC   Cell type: Pancreatic beta cell; CL=CL_0000169.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   8FW
CA   Transformed cell line
DT   Created: 21-03-23; Last updated: 10-04-25; Version: 4
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RX   PubMed=37442376; DOI=10.1016/j.molmet.2023.101772; PMCID=PMC10407753;
RA   Blanchi B., Taurand M., Colace-Sauty C., Thomaidou S., Audeoud C., Fantuzzi F.,
RA   Sawatani T., Gheibi S., Sabadell-Basallote J., Boot F.W.J., Chantier T.,
RA   Piet A., Cavanihac C., Pilette M., Balguerie A., Olleik H., Carlotti F.,
RA   Ejarque M., Fex M., Mulder H., Cnop M., Eizirik D.L., Jouannot O.,
RA   Gaffuri A.-L., Czernichow P., Zaldumbide A., Scharfmann R., Ravassard P.;
RT   "EndoC-betaH5 cells are storable and ready-to-use human pancreatic
RT   beta cells with physiological insulin secretion.";
RL   Mol. Metab. 76:101772.1-101772.13(2023).
//