Cellosaurus cell line CI-huArlo (CVCL

Cellosaurus CI-huArlo (CVCL_E6BF)

[Text version]

Cell line name

CI-huArlo

Synonyms

Arlo

Accession

CVCL_E6BF

Resource Identification Initiative

To cite this cell line use: CI-huArlo (RRID:CVCL_E6BF)

Comments

Characteristics: Derived from parent cell line by single-cell printing and shows an overall improved alveolar phenotype, most notably a tighter barrier formation (InSCREENeX=INS-CI-1031).
Characteristics: While identified as a type 1 pneumocyte (AT-1) under long-term Air-Liquid Interface (ALI) culture conditions, might exhibit type 2 (AT-2) characteristics, including lamellar body formation and surfactant protein secretion (InSCREENeX=INS-CI-1031).
Characteristics: Transduced with a lentiviral library consisting of 33 genes, some of which are retained in the immortalized cell line (CI-SCREEN) (from parent cell line).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5361; ID2.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5362; ID3.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7553; MYC.
Genetic integration: Method=Transduction; Gene=MGI; MGI:88138; Bcl2.
Genetic integration: Method=Transduction; Gene=MGI; MGI:1919200; Nanog.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Genetic integration: Method=Transduction; Gene=Hepatitis C virus core protein.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Lung, alveolus; UBERON=UBERON_0002299.
Cell type: Type 1 pneumocyte; CL=CL_0002062.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_C1M0 (CI-hAELVi)

Sex of cell

Male

Age at sampling

Age unspecified

Category

Transformed cell line

STR profile

Source(s): InSCREENeX=INS-CI-1031

Markers:

Amelogenin	X,Y
CSF1PO	10,13
D2S1338	17
D3S1358	15,17
D5S818	11
D7S820	11
D8S1179	14,15
D13S317	10,12
D16S539	11
D18S51	15,16
D19S433	13,13.2
D21S11	30.2,33.2
FGA	23
TH01	9
TPOX	8
vWA	17,18

Web pages

Provider; InSCREENeX; INS-CI-1031; https://www.inscreenex.de/product/ci-huarlo/

Publications

PubMed=36748276; DOI=10.1002/advs.202207301; PMCID=PMC10015904
Carius P., Jungmann A., Bechtel M., Grissmer A., Boese A., Gasparoni G., Salhab A., Seipelt R., Urbschat K., Richter C., Meier C., Bojkova D., Cinatl J. Jr., Walter J., Schneider-Daum N., Lehr C.-M.
A monoclonal human alveolar epithelial cell line ('Arlo') with pronounced barrier function for studying drug permeability and viral infections.
Adv. Sci. (Weinh.) 10:2207301.1-2207301.23(2023)

Entry history

Entry creation

10-Apr-2025

Last entry update

10-Apr-2025

Version number