ID   CI-huArlo
AC   CVCL_E6BF
SY   Arlo
RX   PubMed=36748276;
WW   Provider; InSCREENeX; INS-CI-1031; https://www.inscreenex.de/product/ci-huarlo/
CC   Characteristics: Derived from parent cell line by single-cell printing and shows an overall improved alveolar phenotype, most notably a tighter barrier formation (InSCREENeX=INS-CI-1031).
CC   Characteristics: While identified as a type 1 pneumocyte (AT-1) under long-term Air-Liquid Interface (ALI) culture conditions, might exhibit type 2 (AT-2) characteristics, including lamellar body formation and surfactant protein secretion (InSCREENeX=INS-CI-1031).
CC   Characteristics: Transduced with a lentiviral library consisting of 33 genes, some of which are retained in the immortalized cell line (CI-SCREEN) (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5361; ID2.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5362; ID3.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7553; MYC.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:88138; Bcl2.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:1919200; Nanog.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
CC   Genetic integration: Method=Transduction; Gene=Hepatitis C virus core protein.
CC   Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
CC   Omics: Transcriptomics; RNAseq.
CC   Derived from site: In situ; Lung, alveolus; UBERON=UBERON_0002299.
CC   Cell type: Type 1 pneumocyte; CL=CL_0002062.
ST   Source(s): InSCREENeX=INS-CI-1031
ST   Amelogenin: X,Y
ST   CSF1PO: 10,13
ST   D13S317: 10,12
ST   D16S539: 11
ST   D18S51: 15,16
ST   D19S433: 13,13.2
ST   D21S11: 30.2,33.2
ST   D2S1338: 17
ST   D3S1358: 15,17
ST   D5S818: 11
ST   D7S820: 11
ST   D8S1179: 14,15
ST   FGA: 23
ST   TH01: 9
ST   TPOX: 8
ST   vWA: 17,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C1M0 ! CI-hAELVi
SX   Male
AG   Age unspecified
CA   Transformed cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=36748276; DOI=10.1002/advs.202207301; PMCID=PMC10015904;
RA   Carius P., Jungmann A., Bechtel M., Grissmer A., Boese A., Gasparoni G.,
RA   Salhab A., Seipelt R., Urbschat K., Richter C., Meier C., Bojkova D.,
RA   Cinatl J. Jr., Walter J., Schneider-Daum N., Lehr C.-M.;
RT   "A monoclonal human alveolar epithelial cell line ('Arlo') with
RT   pronounced barrier function for studying drug permeability and viral
RT   infections.";
RL   Adv. Sci. (Weinh.) 10:2207301.1-2207301.23(2023).
//