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Cellosaurus H1L6.1c3 (CVCL_DC23)

[Text version]
Cell line name H1L6.1c3
Accession CVCL_DC23
Resource Identification Initiative To cite this cell line use: H1L6.1c3 (RRID:CVCL_DC23)
Comments Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
Biotechnology: Used in US Environmental Protection Agency (EPA) hazardous waste test method 4435 (SW-846).
Characteristics: Acts through the activation of a luciferase reporter gene under the control of dioxin-response elements (DREs).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Hepatocellular carcinoma of the mouse (NCIt: C38756)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57L/J.
Hierarchy Parent: CVCL_0328 (Hepa-1c1c7)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
Web pages Info; EPA; -; https://www.epa.gov/hw-sw846/sw-846-test-method-4435-screening-dioxin-chemical-activity-soils-and-sediments-using-calux
Provider; XDSI; -; http://www.dioxins.com/products/
Publications

PubMed=18969542; DOI=10.1016/j.talanta.2004.05.032
Michael S. Denison, Bin Zhao, David S. Baston, George C. Clark, Hiroshi Murata, Dalho Han;
Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals.
Talanta 63:1123-1133(2004)

PubMed=21872055; DOI=10.1016/j.talanta.2011.07.042
Kersten Van Langenhove, Kim Croes, Michael S. Denison, Marc Elskens, Willy Baeyens;
The CALUX bio-assay: analytical comparison between mouse hepatoma cell lines with a low (H1L6.1c3) and high (H1L7.5c1) number of dioxin response elements.
Talanta 85:2039-2046(2011)

PubMed=26366531; DOI=10.1021/acs.est.5b02906; PMCID=PMC4772899
Jennifer C. Brennan, Guo-Chun He, Tomoaki Tsutsumi, Jing Zhao, Edward Wirth, Michael H. Fulton, Michael S. Denison;
Development of species-specific Ah receptor-responsive third generation CALUX cell lines with enhanced responsiveness and improved detection limits.
Environ. Sci. Technol. 49:11903-11912(2015)

Cross-references
Encyclopedic resources Wikidata; Q54871989
Entry history
Entry creation13-Jul-2016
Last entry update14-Aug-2025
Version number13