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Cellosaurus MM2d (CVCL_D5GY)

[Text version]
Cell line name MM2d
Accession CVCL_D5GY
Resource Identification Initiative To cite this cell line use: MM2d (RRID:CVCL_D5GY)
Comments Group: Plant cell line.
Characteristics: Rapid cell proliferation (PubMed=12000456).
Characteristics: Grows in darkness (PubMed=12000456).
Characteristics: The cell cycle can be synchronized by removing and re-supplying sucrose or with aphidicolin (PubMed=12000456; PubMed=16739567).
Characteristics: Can be genetically transformed with Agrobacterium (PubMed=14756764).
Characteristics: Can be used to prepare protoplasts (PubMed=24134884).
Omics: Genomics; ChIP-seq; A.thaliana RBR1.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Stem; PO=PO_0009047.
Species of origin Arabidopsis thaliana (Mouse-ear cress) (NCBI Taxonomy: 3702)
Breed/subspecies: Cultivar Landsberg erecta.
Hierarchy Parent: CVCL_D5GX (MM1)
Children:
CVCL_D5GZ (MM2d-LS)CVCL_D5H0 (PSB-D)
Category Spontaneously immortalized cell line
Publications

PubMed=12231968; DOI=10.1104/pp.103.2.621; PMCID=PMC159023
Mike J. May, Christopher John Leaver;
Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures.
Plant Physiol. 103:621-627(1993)

PubMed=12000456; DOI=10.1046/j.1365-313x.2002.01274.x
Margit Menges, James A.H. Murray;
Synchronous Arabidopsis suspension cultures for analysis of cell-cycle gene activity.
Plant J. 30:203-212(2002)

PubMed=14756764; DOI=10.1046/j.1365-313x.2003.01980.x
Margit Menges, James A.H. Murray;
Cryopreservation of transformed and wild-type Arabidopsis and tobacco cell suspension cultures.
Plant J. 37:635-644(2004)

PubMed=16408177; DOI=10.1007/s00299-005-0075-4
Atsushi Kono, Ryoko Ohno, Chikage Umeda-Hara, Hirofumi Uchimiya, Masaaki Umeda;
A distinct type of cyclin D, CYCD4;2, involved in the activation of cell division in Arabidopsis.
Plant Cell Rep. 25:540-545(2006)

PubMed=16739567; DOI=10.1385/1-59745-003-0:45
Margit Menges, James A.H. Murray;
Synchronization, transformation, and cryopreservation of suspension-cultured cells.
Methods Mol. Biol. 323:45-61(2006)

PubMed=19368694; DOI=10.1111/j.1365-313x.2009.03884.x
Hirotomo Takatsuka, Ryoko Ohno, Masaaki Umeda;
The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation.
Plant J. 59:475-487(2009)

PubMed=20130883; DOI=10.1007/s00299-010-0817-9
Ikuo Takahashi, Shoko Kojima, Norihiro Sakaguchi, Chikage Umeda-Hara, Masaaki Umeda;
Two Arabidopsis cyclin A3s possess G1 cyclin-like features.
Plant Cell Rep. 29:307-315(2010)

PubMed=21613568; DOI=10.1073/pnas.1103584108; PMCID=PMC3116379
Sumiko Adachi, Kazunori Minamisawa, Yoko Okushima, Soichi Inagaki, Kaoru Yoshiyama, Youichi Kondou, Eli Kaminuma, Mika Kawashima, Tetsuro Toyoda, Minami Matsui, Daisuke Kurihara ...Show all 13 authors... , Sachihiro Matsunaga, Masaaki Umeda; Show fewer authors
Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis.
Proc. Natl. Acad. Sci. U.S.A. 108:10004-10009(2011)

PubMed=24134884; DOI=10.1104/pp.113.225607; PMCID=PMC3850192
Takahiro Hamada, Nahoko Nagasaki-Takeuchi, Takehide Kato, Masayuki Fujiwara, Seiji Sonobe, Yoichiro Fukao, Takashi Hashimoto;
Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures.
Plant Physiol. 163:1804-1816(2013)

Cross-references
Cell line collections (Providers) RIKEN_BRC_EPD; rpc00103
Encyclopedic resources Wikidata; Q127382833
Gene expression databases GEO; GSE5747
GEO; GSE5750
GEO; GSE108741
Entry history
Entry creation02-May-2024
Last entry update10-Apr-2025
Version number3