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Cellosaurus HeLa-TetR-Cas9 (CVCL_C9AP)

[Text version]
Cell line name HeLa-TetR-Cas9
Synonyms cTT20
Accession CVCL_C9AP
Resource Identification Initiative To cite this cell line use: HeLa-TetR-Cas9 (RRID:CVCL_C9AP)
Comments Population: African American.
Characteristics: Expression of Cas9 can be induced by doxocycline (PubMed=26698661).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_C9AQ (HeLa RelA-mNeonGreen)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=26698661; DOI=10.1016/j.molcel.2015.10.027; PMCID=PMC4690846
McKinley K.L., Sekulic N., Guo L.Y., Tsinman T., Black B.E., Cheeseman I.M.
The CENP-L-N complex forms a critical node in an integrated meshwork of interactions at the centromere-kinetochore interface.
Mol. Cell 60:886-898(2015)

PubMed=31626775; DOI=10.1016/j.cell.2019.09.016; PMCID=PMC6886477
Feldman D., Singh A., Schmid-Burgk J.L., Carlson R.J., Mezger A., Garrity A.J., Zhang F., Blainey P.C.
Optical pooled screens in human cells.
Cell 179:787-799.e17(2019)

Cross-references
Encyclopedic resources Wikidata; Q123031631
Entry history
Entry creation05-Oct-2023
Last entry update10-Apr-2025
Version number4