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Cellosaurus CEM-GXR25 (CVCL_C8ZB)

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Cell line name CEM-GXR25
Synonyms GXR; CEM-GFP, CXCR4, CCR5, clone 25
Accession CVCL_C8ZB
Resource Identification Initiative To cite this cell line use: CEM-GXR25 (RRID:CVCL_C8ZB)
Comments Population: Caucasian.
Virology: HIV-1 infection reporter cell line, the gene for GFP is under the control of a HIV-1 long terminal repeat. Thansk to the increased expression of CCR5 can be used for both CXCR4-tropic and CCR5-tropic HIV-1 strains (PubMed=16182382).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:1606; CCR5.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; HGNC:13222; BCL11B + HGNC; HGNC:2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1G53 (CEM-GFP)
Children:
CVCL_C8ZC (GXRCas9)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line
Publications

PubMed=16182382; DOI=10.1016/j.jviromet.2005.08.003
Mark A. Brockman, Giancarlo O. Tanzi, Bruce D. Walker, Todd Mackenzie Allen;
Use of a novel GFP reporter cell line to examine replication capacity of CXCR4- and CCR5-tropic HIV-1 by flow cytometry.
J. Virol. Methods 131:134-142(2006)

Cross-references
Encyclopedic resources Wikidata; Q123030896
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number6