ID   CEM-GXR25
AC   CVCL_C8ZB
SY   GXR; CEM-GFP, CXCR4, CCR5, clone 25
DR   Wikidata; Q123030896
RX   PubMed=16182382;
CC   Population: Caucasian.
CC   Virology: HIV-1 infection reporter cell line, the gene for GFP is under the control of a HIV-1 long terminal repeat. Thansk to the increased expression of CCR5 can be used for both CXCR4-tropic and CCR5-tropic HIV-1 strains (PubMed=16182382).
CC   Sequence variation: Gene fusion; HGNC; 13222; BCL11B + HGNC; 2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: HGNC; 1606; CCR5.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1G53 ! CEM-GFP
SX   Female
AG   3Y11M
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 30-01-24; Version: 2
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RX   PubMed=16182382; DOI=10.1016/j.jviromet.2005.08.003;
RA   Brockman M.A., Tanzi G.O., Walker B.D., Allen T.M.;
RT   "Use of a novel GFP reporter cell line to examine replication capacity
RT   of CXCR4- and CCR5-tropic HIV-1 by flow cytometry.";
RL   J. Virol. Methods 131:134-142(2006).
//