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Cellosaurus GXRCas9 (CVCL_C8ZC)

[Text version]
Cell line name GXRCas9
Accession CVCL_C8ZC
Resource Identification Initiative To cite this cell line use: GXRCas9 (RRID:CVCL_C8ZC)
Comments Population: Caucasian.
Transfected with: HGNC; 1606; CCR5.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Omics: CRISPR phenotypic screen.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; 13222; BCL11B + HGNC; 2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C8ZB (CEM-GXR25)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line
Publications

PubMed=27992415; DOI=10.1038/ng.3741
Park R.J., Wang T., Koundakjian D.C., Hultquist J.F., Lamothe-Molina P.A., Monel B., Schumann K., Yu H.-Y., Krupzcak K.M., Garcia-Beltran W.F., Piechocka-Trocha A., Krogan N.J., Marson A., Sabatini D.M., Lander E.S., Hacohen N., Walker B.D.
A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.
Nat. Genet. 49:193-203(2017)

Cross-references
CRISP screens repositories BioGRID_ORCS_Cell_line; 1226
Encyclopedic resources Wikidata; Q123031381
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2