Cellosaurus cell line MSOD-B (CVCL

Cellosaurus MSOD-B (CVCL_C6VX)

Cross-references
Cell line name	MSOD-B
Synonyms	MSOD-BMP2
Accession	CVCL_C6VX
Resource Identification Initiative	To cite this cell line use: MSOD-B (RRID:CVCL_C6VX)
Comments	Characteristics: Capable of chondrogenesis and deposition of a morphogen-enriched ECM (PubMed=34486186). Characteristics: Can successfully undergo chondrogenic and osteogenic differentiation in vitro, forming mature hypertrophic cartilage and osteoid tissues, respectively. Their subsequent ectopic implantation results in rapid humanized ossicles (hOss) formation and reconstitution of a cellular and molecular human mesenchymal niche (PubMed=36223446). Characteristics: Transduced with a lentiviral vector containing hTERT and eGFP respectively under the control of the CMV and EF1a promoters and a retro-vector containing iCas9-2A-CD19t under the control of a CMV promoter (from parent cell line). Characteristics: Transduced with a lentiviral vector containing a PGK bidirectional promoter driving the expression of BMP2 and truncated LNGFR (PubMed=34486186). Characteristics: The suicide system of this cell line can be activated by addition of an homodimerizer that activates the apoptosis pathway through the dimerization of iCas9 (from parent cell line). Transfected with: HGNC; 1069; BMP2. Transfected with: HGNC; 1633; CD19 (with p.Lys324_Arg556del = CD19t). Transfected with: HGNC; 7809; NGFR (truncated). Transfected with: HGNC; 11730; TERT. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371. Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_C6VW (MSOD)
Sex of cell	Female
Age at sampling	51Y
Category	Telomerase immortalized cell line
Publications	PubMed=34486186; DOI=10.1002/adma.202103737 Pigeot S., Klein T., Gullotta F., Dupard S.J., Garcia-Garcia A., Garcia-Garcia A., Prithiviraj S., Lorenzo P., Filippi M., Jaquiery C., Kouba L., Asnaghi M.A., Raina D.B., Dasen B., Isaksson H., Onnerfjord P., Tagil M., Bondanza A., Martin I., Bourgine P.-E. Manufacturing of human tissues as off-the-shelf grafts programmed to induce regeneration. Adv. Mater. 33:e2103737.1-e2103737.13(2021) PubMed=36223446; DOI=10.1126/scitranslmed.abm6391 Grigoryan A., Zacharaki D., Balhuizen A., Come C.R.M., Garcia-Garcia A., Hidalgo Gil D., Frank A.-K., Aaltonen K., Manas A., Esfandyari J., Kjellman P., Englund E., Rodriguez C., Sime W., Massoumi R., Kalantari N., Prithiviraj S., Li Y., Dupard S.J., Isaksson H., Madsen C.D., Porse B.T., Bexell D., Bourgine P.-E. Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia, and solid tumor metastasis. Sci. Transl. Med. 14:eabm6391.1-eabm6391.16(2022)
Encyclopedic resources	Wikidata; Q117704717
Entry history
Entry creation	21-Mar-2023
Last entry update	29-Jun-2023
Version number	2