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Cellosaurus MSOD-B (CVCL_C6VX)

[Text version]
Cell line name MSOD-B
Synonyms MSOD-BMP2
Accession CVCL_C6VX
Resource Identification Initiative To cite this cell line use: MSOD-B (RRID:CVCL_C6VX)
Comments Characteristics: Capable of chondrogenesis and deposition of a morphogen-enriched ECM (PubMed=34486186).
Characteristics: Can successfully undergo chondrogenic and osteogenic differentiation in vitro, forming mature hypertrophic cartilage and osteoid tissues, respectively. Their subsequent ectopic implantation results in rapid humanized ossicles (hOss) formation and reconstitution of a cellular and molecular human mesenchymal niche (PubMed=36223446).
Characteristics: Transduced with a lentiviral vector containing hTERT and eGFP respectively under the control of the CMV and EF1a promoters and a retro-vector containing iCasp9-2A-CD19t under the control of a CMV promoter (from parent cell line).
Characteristics: Transduced with a lentiviral vector containing a PGK bidirectional promoter driving the expression of BMP2 and truncated LNGFR (PubMed=34486186).
Characteristics: The suicide system of this cell line can be activated by addition of an homodimerizer that activates the apoptosis pathway through the dimerization of iCasp9 (FKBP12 linked by a linker SGGGS to Caspase-9) (from parent cell line).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1069; BMP2.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1511; CASP9.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19 (Note=With p.Lys324_Arg556del = CD19t).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3711; FKBP1A (Note=With p.Phe37Val).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7809; NGFR (Note=Truncated).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C6VW (MSOD)
Sex of cell Female
Age at sampling 51Y
Category Telomerase immortalized cell line
Publications

PubMed=34486186; DOI=10.1002/adma.202103737
Pigeot S., Klein T., Gullotta F., Dupard S.J., Garcia-Garcia A., Garcia-Garcia A., Prithiviraj S., Lorenzo P., Filippi M., Jaquiery C., Kouba L., Asnaghi M.A., Raina D.B., Dasen B., Isaksson H., Onnerfjord P., Tagil M., Bondanza A., Martin I., Bourgine P.-E.
Manufacturing of human tissues as off-the-shelf grafts programmed to induce regeneration.
Adv. Mater. 33:e2103737.1-e2103737.13(2021)

PubMed=36223446; DOI=10.1126/scitranslmed.abm6391
Grigoryan A., Zacharaki D., Balhuizen A., Come C.R.M., Garcia-Garcia A., Hidalgo Gil D., Frank A.-K., Aaltonen K., Manas Nunez A., Esfandyari J., Kjellman P., Englund E., Rodriguez-Cupello C., Sime W., Massoumi R., Kalantari N., Prithiviraj S., Li Y., Dupard S.J., Isaksson H., Madsen C.D., Porse B.T., Bexell D., Bourgine P.-E.
Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia, and solid tumor metastasis.
Sci. Transl. Med. 14:eabm6391.1-eabm6391.16(2022)

Cross-references
Encyclopedic resources Wikidata; Q117704717
Entry history
Entry creation21-Mar-2023
Last entry update10-Apr-2025
Version number5