ID   MSOD-B
AC   CVCL_C6VX
SY   MSOD-BMP2
DR   Wikidata; Q117704717
RX   PubMed=34486186;
RX   PubMed=36223446;
CC   Characteristics: Capable of chondrogenesis and deposition of a morphogen-enriched ECM (PubMed=34486186).
CC   Characteristics: Can successfully undergo chondrogenic and osteogenic differentiation in vitro, forming mature hypertrophic cartilage and osteoid tissues, respectively. Their subsequent ectopic implantation results in rapid humanized ossicles (hOss) formation and reconstitution of a cellular and molecular human mesenchymal niche (PubMed=36223446).
CC   Characteristics: Transduced with a lentiviral vector containing hTERT and eGFP respectively under the control of the CMV and EF1a promoters and a retro-vector containing iCas9-2A-CD19t under the control of a CMV promoter (from parent cell line).
CC   Characteristics: Transduced with a lentiviral vector containing a PGK bidirectional promoter driving the expression of BMP2 and truncated LNGFR (PubMed=34486186).
CC   Characteristics: The suicide system of this cell line can be activated by addition of an homodimerizer that activates the apoptosis pathway through the dimerization of iCas9 (from parent cell line).
CC   Transfected with: HGNC; 1069; BMP2.
CC   Transfected with: HGNC; 1633; CD19 (with p.Lys324_Arg556del = CD19t).
CC   Transfected with: HGNC; 7809; NGFR (truncated).
CC   Transfected with: HGNC; 11730; TERT.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
CC   Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C6VW ! MSOD
SX   Female
AG   51Y
CA   Telomerase immortalized cell line
DT   Created: 21-03-23; Last updated: 29-06-23; Version: 2
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RX   PubMed=34486186; DOI=10.1002/adma.202103737;
RA   Pigeot S., Klein T., Gullotta F., Dupard S.J., Garcia-Garcia A.,
RA   Garcia-Garcia A., Prithiviraj S., Lorenzo P., Filippi M., Jaquiery C.,
RA   Kouba L., Asnaghi M.A., Raina D.B., Dasen B., Isaksson H.,
RA   Onnerfjord P., Tagil M., Bondanza A., Martin I., Bourgine P.-E.;
RT   "Manufacturing of human tissues as off-the-shelf grafts programmed to
RT   induce regeneration.";
RL   Adv. Mater. 33:e2103737.1-e2103737.13(2021).
//
RX   PubMed=36223446; DOI=10.1126/scitranslmed.abm6391;
RA   Grigoryan A., Zacharaki D., Balhuizen A., Come C.R.M., Garcia-Garcia A.,
RA   Hidalgo Gil D., Frank A.-K., Aaltonen K., Manas A., Esfandyari J.,
RA   Kjellman P., Englund E., Rodriguez C., Sime W., Massoumi R.,
RA   Kalantari N., Prithiviraj S., Li Y., Dupard S.J., Isaksson H.,
RA   Madsen C.D., Porse B.T., Bexell D., Bourgine P.-E.;
RT   "Engineering human mini-bones for the standardized modeling of healthy
RT   hematopoiesis, leukemia, and solid tumor metastasis.";
RL   Sci. Transl. Med. 14:eabm6391.1-eabm6391.16(2022).
//