Cellosaurus F1G (CVCL_C4MU)
Cell line name | F1G | |
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Accession | CVCL_C4MU | |
Resource Identification Initiative | To cite this cell line use: F1G (RRID:CVCL_C4MU) | |
Comments | Group: Non-human primate cell line. Group: SARS-CoV-2 research cell line. Characteristics: Transduced with a fluorescent reporter (F1G) which is a construct that contains the substrate of the nsp5 protease (SAVLQSGF) between a NLS-eGFP and a membrane anchor. Thus GFP, upon cleavage of the nsp5 protease site by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells (PubMed=36246297). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein. Derived from sampling site: Kidney; epithelium. | |
Species of origin | Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) (NCBI Taxonomy: 60711) | |
Hierarchy | Parent: CVCL_0059 (Vero) Children:
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Sex of cell | Female | |
Age at sampling | Adult | |
Category | Spontaneously immortalized cell line | |
Publications | PubMed=36246297; DOI=10.3389/fmicb.2022.1031204 | |
Entry history | ||
Entry creation | 15-Dec-2022 | |
Last entry update | 15-Dec-2022 | |
Version number | 1 |