ID   F1G
DR   Wikidata; Q116048961
RX   PubMed=36246297;
CC   Group: Non-human primate cell line.
CC   Group: SARS-CoV-2 research cell line.
CC   Characteristics: Transduced with a fluorescent reporter (F1G) which is a construct that contains the substrate of the nsp5 protease (SAVLQSGF) between a NLS-eGFP and a membrane anchor. Thus GFP, upon cleavage of the nsp5 protease site by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells (PubMed=36246297).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC   Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
OX   NCBI_TaxID=60711; ! Chlorocebus sabaeus (Green monkey)
HI   CVCL_0059 ! Vero
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 15-12-22; Last updated: 05-10-23; Version: 4
RX   PubMed=36246297; DOI=10.3389/fmicb.2022.1031204;
RA   Desmarets L.M.B., Callens N., Hoffmann E., Danneels A., Lavie M.,
RA   Couturier C., Dubuisson J., Belouzard S., Rouille Y.;
RT   "A reporter cell line for the automated quantification of SARS-CoV-2
RT   infection in living cells.";
RL   Front. Microbiol. 13:1031204.1-1031204.11(2022).