Cellosaurus F1G-red (CVCL_C4MV)
| Cell line name | F1G-red |
|---|---|
| Accession | CVCL_C4MV |
| Resource Identification Initiative | To cite this cell line use: F1G-red (RRID:CVCL_C4MV) |
| Comments | Group: Non-human primate cell line. Group: SARS-CoV-2 research cell line. Characteristics: Transduced with a fluorescent reporter (F1G) which is a construct that contains the substrate of the nsp5 protease (SAVLQSGF) between a NLS-eGFP and a membrane anchor. Thus GFP, upon cleavage of the nsp5 protease site by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells (PubMed=36246297). Characteristics: Also transduced with a red fluorescent mCherry-NLS construct. Thus SARS-CoV-2 infection can be scored automatically in living cells by comparing the patterns of green and red fluorescence signals (PubMed=36246297). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein. Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819. |
| Species of origin | Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) (NCBI Taxonomy: 60711) |
| Hierarchy | Parent: CVCL_C4MU (F1G) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=36246297; DOI=10.3389/fmicb.2022.1031204 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q116048962 |
| Entry history | |
| Entry creation | 15-Dec-2022 |
| Last entry update | 29-Jun-2023 |
| Version number | 3 |