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Cellosaurus F1G-red (CVCL_C4MV)

[Text version]
Cell line name F1G-red
Accession CVCL_C4MV
Resource Identification Initiative To cite this cell line use: F1G-red (RRID:CVCL_C4MV)
Comments Group: Non-human primate cell line.
Group: SARS-CoV-2 research cell line.
Characteristics: Transduced with a fluorescent reporter (F1G) which is a construct that contains the substrate of the nsp5 protease (SAVLQSGF) between a NLS-eGFP and a membrane anchor. Thus GFP, upon cleavage of the nsp5 protease site by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells (PubMed=36246297).
Characteristics: Also transduced with a red fluorescent mCherry-NLS construct. Thus SARS-CoV-2 infection can be scored automatically in living cells by comparing the patterns of green and red fluorescence signals (PubMed=36246297).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
Species of origin Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) (NCBI Taxonomy: 60711)
Hierarchy Parent: CVCL_C4MU (F1G)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line

PubMed=36246297; DOI=10.3389/fmicb.2022.1031204
Desmarets L.M.B., Callens N., Hoffmann E., Danneels A., Lavie M., Couturier C., Dubuisson J., Belouzard S., Rouille Y.
A reporter cell line for the automated quantification of SARS-CoV-2 infection in living cells.
Front. Microbiol. 13:1031204.1-1031204.11(2022)

Encyclopedic resources Wikidata; Q116048962
Entry history
Entry creation15-Dec-2022
Last entry update29-Jun-2023
Version number3