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Cellosaurus H9 AAVS1-TRE3G-NGN2 (CVCL_C4EK)

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Cell line name H9 AAVS1-TRE3G-NGN2
Accession CVCL_C4EK
Resource Identification Initiative To cite this cell line use: H9 AAVS1-TRE3G-NGN2 (RRID:CVCL_C4EK)
Comments Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (PubMed=34699746).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Children:
CVCL_D1KV (H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1)CVCL_C4EL (H9 AAVS1-TRE3G-NGN2 ATG12-/-)CVCL_D1IJ (H9 AAVS1-TRE3G-NGN2 CCPG1-/-)
CVCL_C4EN (H9 AAVS1-TRE3G-NGN2 FBXO7-/-)CVCL_D1IH (H9 AAVS1-TRE3G-NGN2 GRN-/-)CVCL_E2VU (H9 AAVS1-TRE3G-NGN2 OPTN-/-)
CVCL_C4EM (H9 AAVS1-TRE3G-NGN2 PINK1-/-)CVCL_D1IF (H9 AAVS1-TRE3G-NGN2 RETREG1-/-)CVCL_D1IG (H9 AAVS1-TRE3G-NGN2 RETREG2-/-)
CVCL_D1IA (H9 AAVS1-TRE3G-NGN2 RETREG3-/-)CVCL_D1II (H9 AAVS1-TRE3G-NGN2 TEX264-/-)CVCL_D1KT (H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA)
CVCL_E3KT (H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (heterozygous))
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=34699746; DOI=10.1016/j.molcel.2021.10.001; PMCID=PMC8688335
Alban Ordureau, Felix Kraus, Jiu-Chun Zhang, Heeseon An, Sookhee Park, Tim Ahfeldt, Joao A. Paulo, Jeffrey Wade Harper;
Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagy.
Mol. Cell 81:5082-5098.e11(2021)

Cross-references
Encyclopedic resources Wikidata; Q116049274
Entry history
Entry creation15-Dec-2022
Last entry update19-Dec-2024
Version number5