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Cellosaurus CRMi003-A-3 (CVCL_C1P1)

[Text version]
Cell line name CRMi003-A-3
Synonyms Proliving; GMNN-mScarletI Reporter; LUMCi041-A-3
Accession CVCL_C1P1
Resource Identification Initiative To cite this cell line use: CRMi003-A-3 (RRID:CVCL_C1P1)
Comments Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=36702081).
Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
Characteristics: Using CRISPR/Cas9 a mScarletI-GMNN(1-110) construct was introduced in the AAVS1 safe harbor locus (PubMed=36702081).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Tyr66Trp, p.Ser72Ala, p.Asn146Phe, p.His148Asp, p.Met153Thr, p.Val163Ala, p.Ser175Gly, p.Ala206Lys and p.His231Leu = Turquoise2 (Tq2)).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: mScarlet, a red fluorescent protein.
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B5S5 (CRMi003-A-2)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

PubMed=36702081; DOI=10.1016/j.scr.2023.103031
Arendzen C.H., Cramer S.J., Freund C.M.A.H., Mummery C.L., Ranga A., Mikkers H.M.M.
Introduction of a geminin mScarlet reporter into H2B-mTurq2 hiPSCs for live-cell imaging of proliferation and cell cycling.
Stem Cell Res. 67:103031-103031(2023)

Cross-references
Cell line databases/resources hPSCreg; CRMi003-A-3
Biological sample resources BioSamples; SAMEA110425584
Encyclopedic resources Wikidata; Q114311268
Entry history
Entry creation22-Sep-2022
Last entry update05-Oct-2023
Version number5