Cellosaurus CRMi003-A-3 (CVCL_C1P1)
Cell line name | CRMi003-A-3 |
---|---|
Synonyms | Proliving; GMNN-mScarletI Reporter; LUMCi041-A-3 |
Accession | CVCL_C1P1 |
Resource Identification Initiative | To cite this cell line use: CRMi003-A-3 (RRID:CVCL_C1P1) |
Comments | Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=36702081). Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 a mScarletI-GMNN(1-110) construct was introduced in the AAVS1 safe harbor locus (PubMed=36702081). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Tyr66Trp, p.Ser72Ala, p.Asn146Phe, p.His148Asp, p.Met153Thr, p.Val163Ala, p.Ser175Gly, p.Ala206Lys and p.His231Leu = Turquoise2 (Tq2)). Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transfected with: mScarlet, a red fluorescent protein. Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_B5S5 (CRMi003-A-2) |
Sex of cell | Male |
Age at sampling | <1D |
Category | Induced pluripotent stem cell |
Publications | PubMed=36702081; DOI=10.1016/j.scr.2023.103031 |
Cross-references | |
Cell line databases/resources | hPSCreg; CRMi003-A-3 |
Biological sample resources | BioSamples; SAMEA110425584 |
Encyclopedic resources | Wikidata; Q114311268 |
Entry history | |
Entry creation | 22-Sep-2022 |
Last entry update | 05-Oct-2023 |
Version number | 5 |