ID   CRMi003-A-3
AC   CVCL_C1P1
SY   Proliving; GMNN-mScarletI Reporter; LUMCi041-A-3
DR   BioSamples; SAMEA110425584
DR   hPSCreg; CRMi003-A-3
DR   Wikidata; Q114311268
RX   PubMed=36702081;
CC   Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=36702081).
CC   Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Characteristics: Using CRISPR/Cas9 a mScarletI-GMNN(1-110) construct was introduced in the AAVS1 safe harbor locus (PubMed=36702081).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Tyr66Trp, p.Ser72Ala, p.Asn146Phe, p.His148Asp, p.Met153Thr, p.Val163Ala, p.Ser175Gly, p.Ala206Lys and p.His231Leu = Turquoise2 (Tq2)).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: mScarlet, a red fluorescent protein.
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B5S5 ! CRMi003-A-2
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 22-09-22; Last updated: 05-10-23; Version: 5
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RX   PubMed=36702081; DOI=10.1016/j.scr.2023.103031;
RA   Arendzen C.H., Cramer S.J., Freund C.M.A.H., Mummery C.L., Ranga A.,
RA   Mikkers H.M.M.;
RT   "Introduction of a geminin mScarlet reporter into H2B-mTurq2 hiPSCs
RT   for live-cell imaging of proliferation and cell cycling.";
RL   Stem Cell Res. 67:103031-103031(2023).
//