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Cellosaurus CRMi003-A-2 (CVCL_B5S5)

[Text version]
Cell line name CRMi003-A-2
Synonyms LUMCi041-A-2; DOXiH2BmTurq2
Accession CVCL_B5S5
Resource Identification Initiative To cite this cell line use: CRMi003-A-2 (RRID:CVCL_B5S5)
Comments Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=34775202).
Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (PubMed=34775202).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Tyr66Trp, p.Ser72Ala, p.Asn146Phe, p.His148Asp, p.Met153Thr, p.Val163Ala, p.Ser175Gly, p.Ala206Lys and p.His231Leu = Turquoise2 (Tq2)).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1E71 (NCRM-1)
CVCL_C1P1 (CRMi003-A-3)
Sex of cell Male
Age at sampling <1D
Category Induced pluripotent stem cell

PubMed=34775202; DOI=10.1016/j.scr.2021.102592
Arendzen C.H., Chaudhari U., Cramer S.J., Freund C.M.A.H., Mummery C.L., Ranga A., Pourquie O., Mikkers H.M.M.
Generation of LUMCi041-A-2: equipping a PAX3 reporter iPSC line with doxycycline inducible H2B-mTurquoise2 for live cell imaging.
Stem Cell Res. 57:102592-102592(2021)

Cell line databases/resources hPSCreg; CRMi003-A-2
Biological sample resources BioSamples; SAMEA10199970
Encyclopedic resources Wikidata; Q111733136
Entry history
Entry creation17-Mar-2022
Last entry update29-Jun-2023
Version number4