ID   CRMi003-A-2
AC   CVCL_B5S5
SY   LUMCi041-A-2; DOXiH2BmTurq2
DR   BioSamples; SAMEA10199970
DR   hPSCreg; CRMi003-A-2
DR   Wikidata; Q111733136
RX   PubMed=34775202;
CC   Characteristics: Expresses a doxycycline inducible H2B-mTurquoise2 reporter. Instrumental to track cells and to temporally assess cell divisions and apoptosis in iPSC-based differentiation and cell toxicity assays (PubMed=34775202).
CC   Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-rTA3G-H2B-mTurquoise2-TRE3G construct was introduced in the AAVS1 safe harbor locus (PubMed=34775202).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1E71 ! NCRM-1
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 17-03-22; Last updated: 10-09-24; Version: 5
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RX   PubMed=34775202; DOI=10.1016/j.scr.2021.102592;
RA   Arendzen C.H., Chaudhari U., Cramer S.J., Freund C.M.A.H.,
RA   Mummery C.L., Ranga A., Pourquie O., Mikkers H.M.M.;
RT   "Generation of LUMCi041-A-2: equipping a PAX3 reporter iPSC line with
RT   doxycycline inducible H2B-mTurquoise2 for live cell imaging.";
RL   Stem Cell Res. 57:102592-102592(2021).
//